Legionella Sampling in the Environment A. Legionella Sampling in Air: A1. How to Handle Microbiological Media (AGAR PLATES) Agar plates must be kept refrigerated or in a cooler box with ice packs until ready to use The plates must be allowed to warm up to room temperature before taking a sample (approx. 20 minutes) Do not remove the lid from the plate at anytime … [Read more...] about Legionella Sampling Protocol